mGluR-5 (K56) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2732-50, BS2732-100

Sizes: 50µl, 100µl

Swiss-Prot: P41594

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The mGluR proteins (metabotropic glutamate receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR-1 and mGluR-5 are mediated by a G protein that activates a phosphatidylinositolcalcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight subtypes of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: mGluR5 (K56) polyclonal antibody detects endogenous levels of mGluR5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of mGluR-5 (K56) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: BV2 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (10ug)
Lane 4: SK-OVCAR3 whole cell lysate (40ug)
Lane 5: L02 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of mGluR-5 (K56) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~132 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Metabotropic glutamate receptor 5; mGluR5; GRM5; GPRC1E; MGLUR5

Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human mGluR-5.

Conjugate: Unconjugated

Modification: Unmodified