Topo IIα (E1102) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2643-50, BS2643-100

Sizes: 50µl, 100µl

Swiss-Prot: P11388

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIα and Topo IIβ.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Topo IIα (E1102) polyclonal antibody detects endogenous levels of Topo Iiα protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Topo IIα (E1102) polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate (80ug)
Lane 2: HEK293T cell lysate (80ug), Immunohistochemistry (IHC) analyzes of Topo IIα (E1102) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 174 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Topoisomerase II alpha 170 kDa; Topo II alpha; Topoisomerase DNA II alpha 170kDa; TopII alpha; TP2A; TOP2A; TOP2; TOP 2A; EC 5.99.1.3; DNA topoisomerase II alpha isozyme; DNA topoisomerase II 170 kD; DNA topoisomerase 2 alpha; DNA Topoisomerase2

Immunogen: Synthetic peptide, corresponding to amino acids 1071-1220 of Human Topo IIα.

Conjugate: Unconjugated

Modification: Unmodified