Phospho-EphA3-Y779 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74165-50, BS74165-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29320

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T and Hela cells, using Phospho-EphA3-Y779 antibody at 1:2000 dilution. 293T cells were treated by Pervanadate for 30 minutes after serum-starvation overnight. HeLa cells were treated by Pervanadate for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 110kDa

Specificity: Phosphorylated

Alternative Name: EK4; ETK; HEK; ETK1; HEK4; TYRO4; EPHA3

Immunogen: A synthetic phosphorylated peptide around Y779 of human EphA3(NP_005224.2).

Conjugate: Unconjugated

Modification: Phosphorylated