Recombinant Human IL2Ra/CD25 Protein (Fc Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032570-10, PKSH032570-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: IL2Ra; CD25

Target Symptom: Interleukin-2 receptor subunit alpha; CD25; p55; TAC antigen; IL2-RA; IL-2R subunit alpha; IL-2-RA; IL-2 receptor subunit alpha; IL-2Rα; IDDM10; IL2R; p55; TCGFR

Research Area: immunology

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc

UNIProt ID: P01589

Accession: P01589

Background: Interleukin-2 receptor subunit alpha (IL2RA) is a single-pass type I membrane protein; contains 2 Sushi (CCP/SCR) domains. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains; together with the common gamma chain (IL2RG); constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor; while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein; soluble IL2RA has been isolated and determined to result from extracellular proteolyisis.

Activity: Immobilized Human IL-2RA-Fc at 10μg/ml(100 μl/well) can bind Human IL-2-His(Cat: PKSH032629). The ED50 of Human IL-2RA-Fc is 0.46 ug/mL

Sequence: Glu22-Cys213

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 48.9 kDa

Observed MW: 60-90 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only