Recombinant Human ACE2 Protein (Fc Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSR030492-10, PKSR030492-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: ACE2

Target Symptom: Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2

Research Area: Cell biology; Cardiovascular

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc

UNIProt ID: Q9BYF1

Accession: Q9BYF1

Background: Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.

Activity: Immobilized Human ACE-2-Fc(Cat#PKSR030492) at 2μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-mFc(Cat#PKSR030476). The ED50 of Recombinant 2019-nCoV S Protein RBD-mFc(Cat#PKSR030476) is 15.5 ng/ml.

Sequence: Gln18-Ser740

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 1 mM ZnCl2, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 110.5 kDa

Observed MW: 110-140 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only