CDK4 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10716-50, MB10716-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P11802

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 34 kDa; Observed MW: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdk4 in K562, C6, 3T3, Hela lysates using CDK4 antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk4 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: CMM3; PSK-J3

Immunogen: Recombinant protein of human Cdk4

Conjugate: Unconjugated

Modification: Unmodified