IL-16 (G97) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3690-50, BS3690-100

Sizes: 50µl, 100µl

Swiss-Prot: Q14005

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IL-16 (G97) polyclonal antibody detects endogenous levels of IL-16 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IL-16 (G97) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of IL-16 (G97) pAb in paraffin-embedded human liver carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 142 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Pro-interleukin-16; IL16

Immunogen: Synthetic peptide, corresponding to amino acids 66-122 of Human IL-16.

Conjugate: Unconjugated

Modification: Unmodified