PIST Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91076-50, BS91076-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9HD26(Human) Q8BH60(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IF, IP, IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: PIST (PDZ protein interacting specifically with TC10), also known as GOPC (golgi associated PDZ and coiled-coil motif containing), CAL or FIG, is a 462 amino acid protein that localizes to the cytoplasm, as well as to the membrane of the golgi apparatus and to the cell junction. Expressed ubiquitously and containing one PDZ (DHR) domain, PIST functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, PIST is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding PIST are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Three isoforms of PIST exist due to alternative splicing events.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: PIST polyclonal antibody detects endogenous levels of PIST protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution., ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PIST, GOPC, CAL, FIG, CAL, CFTR associated ligand, CFTR-associated ligand, dJ94G16.2, dJ94G16.2 PIST, FIG, Fused in glioblastoma, Golgi associated PDZ and coiled coil motif containing, Golgi associated PDZ and coiled coil motif containing protein, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC 1, GOPC, GOPC, GOPC1, OTTHUMP00000040403, PDZ protein interacting specifically with TC 10, PDZ protein interacting specifically with TC10, PDZ/coiled coil domain binding partner for the rho family GTPase TC 10, PDZ/coiled coil domain binding partner for the rho family GTPase TC10, PIST, Protein interacting specifically with Tc 10, Protein interacting specifically with Tc10,

Immunogen: Recombinant protein within human PIST aa 1-100.

Conjugate: Unconjugated

Modification: Unmodified