ATP5A1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS8506-50, BS8506-100

Swiss-Prot: P25705

Host: Rabbit

ATP5A1 Polyclonal Antibody-BS8506

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200

Background: Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes, F1, the hydrophilic catalytic core, and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one ε chain. F0 consists of three subunits, a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ATP5A1 polyclonal antibody detects endogenous levels of ATP5A1 protein.

Bioworld Molecular Weight: ~ 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATP5A1 polyclonal antibody at 1:500 dilution
LaneA:HEK293T whole cell lysate (40ug)
LaneB:Lela whole cell lysate (40ug)
LaneC:H466 whole cell lysate (40ug)
LaneD:The Lung tissue lysate of Mouse (40ug)
LaneE:The Liver tissue lysate of Mouse (40ug)
LaneF:The Lung tissue lysate of Rat (40ug)
LaneG:The Liver tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of ATP5A1 pAb in paraffin-embedded human breast cancer tissue at 1:50.

Alternative Name: ATP synthase subunit alpha, mitochondrial; ATP5A1; ATP5A; ATP5AL2; ATPM

Immunogen: Synthetic peptide, corresponding to Human ATP5A1.

Modification: Unmodified

Conjugate: Unconjugated