CD22 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10685-50, MB10685-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P20273

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Background: Acts as a regulator of B cell signaling. CD22 is expressed as both a cytoplasmic and membrane protein during discrete stages of B cell lymphocyte differentiation. The cytoplasmic form of CD22, expressed early in B cell development, is a useful marker for acute lymphocytic leukemia. The membrane form of CD22 is expressed in mature B cells prior to their differentiation into plasma cells. Alternative splicing results in two different isoforms, CD22α and CD22β.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 95 kDa; Observed MW: 140 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of CD22 in HL-60 using CD22 antibody, and DAPI., Western blot analysis of CD22 in Raji, K562, Ramos lysates using CD22 antibody.

Alternative Name: CD22; SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD antigen CD22

Immunogen: Recombinant protein of human CD22

Conjugate: Unconjugated

Modification: Unmodified