Phospho-PAK1-T212 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74153-50, BS74153-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13153

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-PAK1-T212 pAb at 1:1000 dilution. 293T cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 55KDa

Specificity: Phosphorylated

Alternative Name: PAK1; PAKalpha

Immunogen: A synthetic phosphorylated peptide around T212 of human PAK1(NP_002567.3).

Conjugate: Unconjugated

Modification: Phosphorylated