Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from mammalian blood by density gradient centrifugation.Density 1, 077 g/ml.Best alternative to Ficoll^®.

ROTI^®Sep 1077 animal excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ration of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with non-coagulated peripheral whole blood from all mammals. In all respective protocols originally using Ficoll^® this reagent may be replaced by ROTI^®Sep 1077 animal without any adjustment.