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Tris Glycine Transfer Buffer 10X sterile stock solution.
Dilute 100ml Tris Glycine Transfer Buffer 10X with 900 ml deionised water to make 1 litre of Tris Glycine Buffer.
Final concentration is 25 mM Tris Base, 0.192 M Glycine and 1g/l SDS.
pH, 20 C. 8.45 ± 0.01 at 1 x use rate
Final concentration after diluting
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| TRIS | 0.25 | M | 30.3 | g/L |
| Glycine | 1.92 | M | 144.1 | g/L |
| SDS | 10.0 | g/L |
Store at room temperature
Keep away from light
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