Vegetal LoFlo MediumLoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to
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LoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long-term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to <24 h but allowing HL-5-containing endosomes to disappear, reducing autofluorescence.